You are performing a PCR reaction but unbeknownst to you, there is a significant pool of dUTP in the nucleotide mix (alo

[answerhappygod]

You are performing a PCR reaction but unbeknownst to you, thereis a significant pool of dUTP in the nucleotide mix (along withdCTP, dTTP, dATP, and dGTP). How might this affect your PCRproduct?
a.
Uracil would be incorporated into the product and would lessenthe affinity of any DNA binding proteins that might bind to theproduct in subsequent experiments.
b.
If the pool of dTTP ran out before the pool of dUTP, DNAreplication could no longer occur.
c.
Nothing would happen since polymerases can't use dUTP to makeDNA
d.
If the PCR product was ligated into a plasmid and put into acell, a totally different mRNA would be made from the insertcompared to an insert made with T's.
e.
During the reaction, uracils incorporated into the product wouldcause the PCR product to degrade as it is being made.