I. DETERMINING GENE ORDER We want to map the distance between genes E (tall height), B (Blue flower color), and G (thick

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I Determining Gene Order We Want To Map The Distance Between Genes E Tall Height B Blue Flower Color And G Thick 1 (227.81 KiB) Viewed 46 times

I. DETERMINING GENE ORDER We want to map the distance between genes E (tall height), B (Blue flower color), and G (thick stalk). Each gene has a recessive allele (e= short, b=white and g=thin). (tester) Your testcross genotypes (use slash notation) will be. Note: The actual configuration of the trihybrid will be deduced as part of the process. Table 1. Test-cross progeny phenotypes #s: 1) Tall, white, thin: 8 4) Tall, blue, thin: 120 7) Short, white, thin: 147 Based on these results, are the genes linked or not? 2) Tall, white, thick: 498 Based on the results above, the parental progeny can be observed in the phenotypes #s: 2 and their corresponding genotypes (use slash notation) are: EbG/ebg and Centromers E E e e 5) Tall, blue, thick: 149 8) Short, blue, thin: 500 G G X The actual configuration of the parental chromosomes in the trihybrid plant is (use slash notation): The double crossover progeny phenotypes #s are: 1 and (use slash notation) are: Ebg/ebg and their corresponding genotypes and 3) Short, white, thick: 118 6) Short, blue, thick: 7 Compare the double crossover classes with the parental classes. Based on this comparison, the actual gene order is: Complete the figure (genes are located on the long arm of the chromosomes): Trihybrid parental chromosomes b b B B g X For the remaining questions in this Activity ignore the male gamete when writing the genotypes Rewrite the genotype of the mating results from Table 1 with the genes in the correct order: 1) Egb EGb_ 2) 3) eGb 4)___EgB_ 8) 5)_ 6) 7)_ Double crossover recombinants and II. MAP DISTANCE To figure out the distance, we must identify the single crossover classes (write as genotypes). The single crossover classes are: __eGb_ EgB Identify the offspring classes that have recombined between the G and E genes. These are the single crossovers eGb and as well as the double crossovers Egb and Distance between G and E = (Recomb. Freq.) (distance) Identify the offspring classes that have recombined between the G and B genes. These are the single crossovers EGB and as well as the double crossovers Egb and Distance between G and B = Distance between E and B = FINAL MAP (include ALL 3 distances): = (Recomb. Freq.) (distance) }