Transcription and Translation 1. Obtain a DNA sequence from your instructor. 2. Copy each base from the sequence into column 1 of the table on your answer sheet. 3. Replicate the sequence using the base pairing rules and write the new strand's sequence in column 2 of the table on your answer sheet. 4. Use column 2 as the template strand to transcribe your DNA sequence into RNA. Record your mRNA sequence in column 3 of the table on your answer sheet. 5. Use the genetic code to match the 3-base codons from your mRNA to the appropriate amino acids. 6. Record your amino acid sequence in column 4 of the table on your answer sheet. First letter A U UUU UUC Phe UUA UUG Leu CUUT CUC CUA CUG UCU UCC UCA Leu UCG Leu AUU AUC ile GUUT GUC GUA GUGJ AUA AUG Met Val C Second letter CCUT CCC CCA CCG] ACU ACC ACA ACG GCU GCC GCA GCG Ser Pro Thr Ala A CAUT CACJ UAU Tyr CYS UAC. UAA Stop UGA Stop UAG Stop UGG Trp His CAA CAG} Gin AAC Asn AAA Lys AAG LYS GAU GACJ G Asp GAA GAG Glu UGU UGC CGUT CGC CGA CGGJ Arg AGU AGC AGA AGGJ -Ser Arg GGUN GGO GGA GGGJ SCAG Gly SURO SFAC G Third letter
11. Using the DNA sequence provided by your instructor, fill in the table below. Enter the DNA sequence from your instructor into Column 1. Simulate DNA replication by making a new DNA strand in Column 2 that would form base-pairs with the DNA in Column 1. Simulate transcription by making an mRNA strand in Column 3 that would form base- pairs with the DNA in Column 2. Using the codon table in the Lab Instructions, decode the mRNA into an amino acid sequence and enter the results in Column 4. Replication, Transcription, and Translation Table Column 3 Column 4 1 2 3 4 5 6 7 a 30 9 10 11 12 13 14 15 16 17 18 Column 1 DNA sequence from instructor Column 2 New DNA strand mRNA strand Amino acid sequence
Coding Strand: S'TAG CTATA AGT C Template Strand & 3/ mRNA transcript 5¹ translation sequence: طاطانه
Transcription and Translation 1. Obtain a DNA sequence from your instructor. 2. Copy each base from the sequence into co
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Transcription and Translation 1. Obtain a DNA sequence from your instructor. 2. Copy each base from the sequence into co
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