- Need Help With Filling In The Chart Based On The Gel Please 1 (1.56 MiB) Viewed 41 times
Band 1 (top) 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 TABLE for calculating DNA fragment sizes (see instructions above): Lane 3 or 10 (1kb ladder) Distance Actual traveled size (kb) 12 0.1 Lane 5 (PCR Fragment) Distance SIZE traveled Lane 7 (Insert restriction) Distance SIZE traveled Lane 8 (vector control) Distance SIZE traveled Lane 9 (vector restriction) Distance SIZE traveled
1Kb DNA Ladder ፐ ፐ 2,000 ← 1,650 ↑↑ ↑ up 12,000 ↑ ↑ ↑ ↑ 5,000 G- 1,000 850 650 500 400 300 200 100 1 Kb Plus DNA Ladder (Invitrogen) 0.7 µg/lane 0.9% agarose gel stained with ethidium bromide Structure of Fragments in 1-Kb Increments: 5'-GATCC-- G -CCTAG-5' (996 bp),