The concentration of acetylcholine (a neurotransmitter) in a sample can be determined from the pH changes that accompany

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The concentration of acetylcholine (a neurotransmitter) in asample can be determined from the pH changes that accompany itshydrolysis. When the sample is incubated with the enzymeacetylcholinesterase, acetylcholine is converted to choline andacetic acid, which dissociates to yield acetate and a hydrogen ion.In a typical analysis, 10 mL of an aqueous solution containing anunknown amount of acetylcholine had a pH of 7.65. When incubatedwith acetylcholinesterase, the pH of the solution decreased to6.87. Assuming there was no buffer in the assay mixture, determinethe number of moles of acetylcholine in the 10 mL sample.