Consider The Data From Yeast Two Hybrid And Co Ip Experiments The Yeast Was Grown On Media With X Gal So That If Lacz 1 (327.73 KiB) Viewed 87 times
Consider the data from yeast-two-hybrid and Co-IP experiments. The yeast was grown on media with X-gal so that if LacZ (recall from Unit 4, lac operon) is active then the colonies will be BLUE and if it is not active the the colonies will be yellow. Sampang These cells had GAL4-AD and GAL4-BD attached to proteins known not to interact. chuy GAL4-AD+Q1 GAL4-BD + Q2 These cells had GAL4-AD and GAL4-BD attached to proteins known to interact. GAL4-AD+Q1 GAL4-BD+Q3 Campin whis GAL4-AD+Q2 GAL4-BD+Q3 Tagged protein: Q2-tag-(BEAD) → Q1 present? (1) Q3 present? GAL4-AD+Q2 GAL4-BD + Q4 (11) Q4 present? (III)
a.) What sequence is GAL4 binding to inside of the yeast cells? (0.5 pt) b.) What is the reporter gene used in this experiment? (0.5 pt) c.) Based on the yeast two hybrid data, which proteins are interacting? The proteins are Q1, Q2, Q3 and Q4. (2 pts) d.) Imagine you did a coimmunoprecipitation experiment where you used tagged protein Q2, isolated it on a bead and then tested to see if a band showed up. Using the yeast-two hybrid data as a guide, predict whether (I), (II) and/or (III) will have a band. (2 pts.)
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