for anion exchange chromatography, what is the best answer? -The buffer was at a lower pH during washing in order to enc

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answerhappygod
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for anion exchange chromatography, what is the best answer? -The buffer was at a lower pH during washing in order to enc

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for anion exchange chromatography, what is the best answer?
-The buffer was at a lower pH during washing in order toencourage LDH binding.
-The buffer with a higher pH enabled binding of LDH with a pI of6.3 (reference), promoting binding.
-The buffers and matrix Sepharose Q that the LDH protein hadbound were eluted.
-The buffer was at a pH higher than the pI, allowing fractionsto elute while promoting LDH binding.
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