You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and

[answerhappygod]

You treat some cancer cells with a new therapeutic that you hopewill kill them. You run an MTT assay on the results and notice thatthe sample from the control cells (not treated with drug) turnspurple upon addition of the MTT and the sample from the cellstreated your drug are clear after adding MTT. As you walk down thehall to run your sample on the spectrophotometer, how are youfeeling? (a) frustrated, (b) unsure, (c) excited.
I understand that if the cell are alive, they will havefunctioning mitochondria, the mitochondria will be producing NADHand NADPH which will allow some enzymes in the cell to convertempty T which is colorless, into a colored form as and die. Thecolorelss means less dye with present, which means the less empty Twas converted, meaning that we had less mitochondrial activity,which means we had less live cells. I think that the answer shouldbe (a) frustrated.
I am not sure if I am right. Glad if the expert advise.