Figure 3:Expression of specific mRNAs and proteins in the Sam68-/- or Sam68+/+ spermatogonial cells. Spag16 mRNA B 1.0 5

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Figure 3 Expression Of Specific Mrnas And Proteins In The Sam68 Or Sam68 Spermatogonial Cells Spag16 Mrna B 1 0 5 1 (68.86 KiB) Viewed 19 times

Figure 3:Expression of specific mRNAs and proteins in the Sam68-/- or Sam68+/+ spermatogonial cells. Spag16 mRNA B 1.0 50.8 mRNA levels (% of expression in wt) 0.6 0.4 0.2 0.0L mRNA levels (% of expression in wt) polys. RNPS Tpn2 mRNA 1.0 0.8 0.6 0.4 0.2 0.0L mRNA levels (% of expression in wt) 0.8 0.6 0,4 0.2 0.0 Nedd1 mRNA polys. RNPS mRNA levels (% of expression in w 1.2 0.9 0.6 0.3 0.0L mRNA levels (% of expression in wt) Prm2 mRNA 1.0 0.8 0.6 0.4 0.2 0.0L Spdya mRNA polys. Sam68** Samo RNPS с kD 70- 95 70 34- 42- Sam68** Sam68 Sam68 SPAG16 NEDD1 SPDYA ERK2 germ cell extracts polys. RNPS polys. RNPS (B) Quantitative real-time PCR analysis of Spag16, Nedd1, Spdya, Tpn2, and Prm2 mRNA levels in the polysomal and ribonucleoprotein (RNP) fractions of spermatogonial cells. (C) Western blot analysis of Sam68, SPAG16, NEDD1, and SPDYA proteins in germ cells isolated from Sam68+/+ or Sam68-/- testes. ERK2 was used as a loading control. Polys polysome, RNP= ribonucleoprotein