Box Or Shield To Cover Lamp 3 Nutrient Agar Plates Inoculating Loop Or Swab Bunsen Burner And Striker Procedure Pe 1 (46.61 KiB) Viewed 30 times
Box Or Shield To Cover Lamp 3 Nutrient Agar Plates Inoculating Loop Or Swab Bunsen Burner And Striker Procedure Pe 2 (32.1 KiB) Viewed 30 times
Box Or Shield To Cover Lamp 3 Nutrient Agar Plates Inoculating Loop Or Swab Bunsen Burner And Striker Procedure Pe 3 (30.63 KiB) Viewed 30 times
Box Or Shield To Cover Lamp 3 Nutrient Agar Plates Inoculating Loop Or Swab Bunsen Burner And Striker Procedure Pe 4 (18.31 KiB) Viewed 30 times
Box Or Shield To Cover Lamp 3 Nutrient Agar Plates Inoculating Loop Or Swab Bunsen Burner And Striker Procedure Pe 5 (22.71 KiB) Viewed 30 times
• Box or shield to cover lamp . 3 nutrient agar plates • Inoculating loop or swab Bunsen burner and striker Procedure Period 1 1. Use your marker to divide each plate in half (mark the bottom of t the plate as shown in figure 24.2a). 2. Label one side of the plate "covered" and the other side "uncovered." As an alternative, your instructor may have you make separate plates for "covered" and "uncovered. If you use separate plates, you will not need index cards. 3. Label one plate "A one "B." and one "C" 4. Use a loop to streak each sample of bacteria on half of each plate (as shown in figure 24.2b). 5. Place plate A under the UV (figure 24.3a). Remove the lid from the plate and cover half of the plate with an index card (as i shown in figure 24.3b). Turn on the lamp and expose the plate for 30 seconds. Make sure you are wearing goggles and do not have your hands under the lamp. 6. Expose plate B for 1 minute. Expose plate C for 5 minutes. 7. Your professor may assign your group different times than the ones listed here. 8. Put the lids back on your plates and put the plates in the incubator lid side down 9. The incubator must be dark (to prevent light repair), and you should put the plates into the incubator as soon as possible. Period 2 1. Examine your plates for growth on each side. 2. Record your results. 31 GALLON Index card (0) (b) Figure 24.3 (a) Placing the plate under the UV lamp: (b) covering the plate with an index card. Uncovered Semania Covered Bacilus (b) Figure 24.2 Preparing the plates. (a) Labeling a plate; (b) pattern for streaking the plates.
8 Results and Interpretation Your three plates may or may not look the same as one another and the plates shown here (figure 24.4). Compare the growth on the covered side to the uncovered side (or covered and uncovered plates) at the various exposure times and also compare the growth of Bacillus to that of Serratia. ww s Min (a) (0) (c) Figure 24.4 Growth of two different bacteria on a plate exposed to (a) no UV light, (b) UV light for a moderate amount of time, and (c) UV light for a longer amount of time. The top half of the plates shown in (b) and (c) were exposed to UV light. The bottom portions of these plates were covered and not exposed to UV.
Interpretation and Questions 1. Which time was the most effective? Least effective? 2. What difference would you expect to see in the results using an older Bacillus culture versus a younger one. Why? 3. Did you see a difference between the survival of the two types of bacteria when exposed to UV light for various times? What do you think is the reason?
4. What would you expect to happen if you forgot to remove the lids on all your plates when you put them under the UV lamp? Why?
Lab results 000000
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