- Give Equation And Steps Please 1 (72.9 KiB) Viewed 35 times
Give equation and steps Please.
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answerhappygod
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Give equation and steps Please.
Give equation and steps Please.
3) Electrical activity of an approximately spherical neuron in culture was recorded with intracellular electrodes. The experiments were carried out at 37° C. The normal saline bathing the cell contained 145 mM Na+ and 5.6 mM K+. In this normal saline the resting potential was measured to be -46 mV, but if the Na+ was replaced with an impermeant ion (i.e. the cell was bathed in a Na-free solution) the resting potential increased to -60 mV. You may assume that in normal saline the ENa was +35 mV, that the membrane is impermeable to CI-, that Ca++ is not directly involved, and to simplify the issue you can ignore Na efflux in the Na- free saline condition (i.e. assume the cell is not permeable to Na in that condition). a. Estimate the intracellular Na+ and K+ concentrations. b. What ratio of gna to gk would account for the normal resting potential? Hint: equal currents... c. In the normal saline condition, if you suddenly made the cell more permeable to K+, what would happen to cell's membrane potential? At what value would it stabilize? d. Starting from the initial normal saline condition, if you added KCl to the extracellular solution so the external K+ concentration became 56 mM, what would the cell's resting potential become?
3) Electrical activity of an approximately spherical neuron in culture was recorded with intracellular electrodes. The experiments were carried out at 37° C. The normal saline bathing the cell contained 145 mM Na+ and 5.6 mM K+. In this normal saline the resting potential was measured to be -46 mV, but if the Na+ was replaced with an impermeant ion (i.e. the cell was bathed in a Na-free solution) the resting potential increased to -60 mV. You may assume that in normal saline the ENa was +35 mV, that the membrane is impermeable to CI-, that Ca++ is not directly involved, and to simplify the issue you can ignore Na efflux in the Na- free saline condition (i.e. assume the cell is not permeable to Na in that condition). a. Estimate the intracellular Na+ and K+ concentrations. b. What ratio of gna to gk would account for the normal resting potential? Hint: equal currents... c. In the normal saline condition, if you suddenly made the cell more permeable to K+, what would happen to cell's membrane potential? At what value would it stabilize? d. Starting from the initial normal saline condition, if you added KCl to the extracellular solution so the external K+ concentration became 56 mM, what would the cell's resting potential become?