Blood typing may be performed safely and quickly by using a test kit. Test kits contain antisera, which cause agglutinat

[answerhappygod]

Blood typing may be performed safely and quickly by using a test kit. Test kits contain antisera, which cause agglutination in non-compatible blood types. The antisera are usually mixtures that contain antibodies from mammals other than humans, and have been tested to work effectively with human blood. You will perform blood typing on glass slides or on test cards, whichever your instructor provides. The blood sample may be provided by your instructor, and may be an artificial blood substitute or animal blood. Alternatively, your instructor may authorize you to test your own blood. Whether using your own blood or that of an animal source, follow the safety precautions carefully: Safety precaution: Wear disposable gloves and eye protection while performing blood tests. At the conclusion of the lab, place all contaminated materials (gloves, slides, toothpicks, etc.) into a biohazardous waste container, wipe down the countertops with 10% bleach solution, and thoroughly wash your hands with soap and water. If an accident occurs, notify your instructor immediately.
1. Obtain two clean glass slides (or test cards), anti-A, anti-B, and anti-Rh typing sera, unused toothpicks, a marking pencil, and an Rh typing box. If you will be testing your own blood, also obtain a sterile lancet, alcohol swabs, and a medicine dropper.2. Divide one slide into two halves with the marking pencil. On a small corner of the area, label half of the slide “anti-A” , then place one drop of anti-A serum onto the center of the half slide. Label “anti-B” on the other half of the slide, using a small corner of the area only, then place one drop of anti-B serum onto its center. Place one drop of blood onto the drop of anti-A serum, and a drop of blood onto the anti-B serum.3. Use a clean toothpick to mix the anti-A serum with the blood. Use a clean, unused toothpick to mix the anti-B serum with the blood (do not use the same toothpick for both samples!).4. Allow 2 minutes for the reaction to occur, then observe your samples for agglutination (clumping or the presence of granulation), and record your results in the Data Table provided. Use Figure 4.2 as a reference.5. Label the second slide “anti-Rh” on a small corner only, then place one drop of anti-Rh serum onto its center. Place a drop of blood onto the anti-Rh serum, then mix with a clean, unused toothpick.6. Place the slide in the Rh typing box to warm, and rock it gently to push the reaction.7. Allow 2 minutes for the reaction to occur, then observe your sample for agglutination (clumping or the presence of granulation), and record your results in the Data Table provided.8. Clean-up: All used toothpicks, glass slides and lancets/needles should be put into appropriate disposal containers. All other materials may be thrown in garbage or placed in green trays for washing. Clean lab benches with 10% bleach solution.
Based on the experiment that you performed above, complete the following table. B I X. X Ω· Undo C Start Over Table Title Data Table: Blood Typing Column 1 Agglutination Results Clumping with anti-A (Yes/No) Clumping with anti-B (Yes/No) Clumping with anti-Rh (Yes/No) ABO blood type of sample: Rh blood of sample: What blood types can you safely donate blood to? What blood types can you safely receive blood from? Column 2 Response Column Row