Results 1 0.8- 0.6 0.4 Absorbance Scan 0.2 0 200 220 Sample dsDNA 240 Well 260 230 A1 260 280 300 320 0.3296 0.1631 0.16
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Results 1 0.8- 0.6 0.4 Absorbance Scan 0.2 0 200 220 Sample dsDNA 240 Well 260 230 A1 260 280 300 320 0.3296 0.1631 0.16
Questions: 1. What is the light path length used in this instrument for the measurement? (tip: use the equation from Beer-Lambert Law) 2. Calculate the missing values for the measurements 3. Comment on the purity of nucleic acid samples.
Results 1 0.8- 0.6 0.4 Absorbance Scan 0.2 0 200 220 Sample dsDNA 240 Well 260 230 A1 260 280 300 320 0.3296 0.1631 0.1686 329.62 340 360 380 280 ng/μl Ratio 260/230 Ratio 260/280 ? 400 ? 1 0.8 0.6 0.4 0.2 Absorbance Scan 200 220 Sample RNA 240 260 Well 260 230 C1 280 280 0.0188 0.0117 0.0100 300 320 340 360 380 400 ng/μl Ratio 260/230 Ratio 260/280 ?... ?... ...?.