4 You Have Discovered A Novel Plasma Membrane Protein That You Think Is A Receptor For Rose Smell In Bees You Obtain I 1 (69.71 KiB) Viewed 32 times
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4 You Have Discovered A Novel Plasma Membrane Protein That You Think Is A Receptor For Rose Smell In Bees You Obtain I 2 (37.14 KiB) Viewed 32 times
4. You have discovered a novel plasma membrane protein that you think is a receptor for rose smell in bees. You obtain its amino acid sequence and determine that a full-length precursor of this protein can be divided into five regions (a-e in the diagram below; N and C indicate amino and carboxy termini). Your biochemical analysis shows that regions b and d are comprised of hydrophobic amino acids and likely serve as internal start and stop transfer sequences for co-translational insertion into the membrane of the endoplasmic reticulum. Using this knowledge, answer the questions below. (3 points each, 9 points total) a b C d e N A. When this receptor appears on the plasma membrane, is the N terminus of this protein located in the cytoplasm or outside of the cell?_ B. How about its C terminus? (in the cytoplasm or outside?). C. Which region(s) of this protein is(are) most likely to glycosylated? - C 5. Determine whether the proteins below (we studied these in class) perform uniport, symport, or antiport function. Indicate whether they carry out active or passive transport, and where in the animal cell these proteins are typically located. (2 points each, 12 points total) Na+pump a) uniport/symport/antiport? b) active/passive transport? c) location: pyruvate transporter a) uniport/symport/antiport? b) active/passive transport? c) location:
7. You have isolated a yeast protein that normally resides in the lumen of the endoplasmic reticulum, and found that this protein has a specific sequence of four amino acids at its carboxy terminus that you suspect functions as an ER retention signal. A. What experiment would demonstrate that this sequence is necessary for retaining this protein in the ER? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (2 points each; 4 total) Experiment: Expected outcome: B. What experiment would demonstrate that this sequence is sufficient for retaining this protein in the ER? Describe the experiment in one sentence, and indicate the expected outcome in the second sentence. (2 points each; 4 total) Experiment: Expected outcome:
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