Name: BIO2073: Microbiology Lab Lab Report Selective and Differential Media: Mannitol Salt Agar, MacConkey Agar, EMB aga

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Name: BIO2073: Microbiology Lab Lab Report Selective and Differential Media: Mannitol Salt Agar, MacConkey Agar, EMB aga

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Name Bio2073 Microbiology Lab Lab Report Selective And Differential Media Mannitol Salt Agar Macconkey Agar Emb Aga 1
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Name: BIO2073: Microbiology Lab Lab Report Selective and Differential Media: Mannitol Salt Agar, MacConkey Agar, EMB agar 1. What was the objective of this lab? 2. For the Mannitol Salt Agar plates, fill in the chart below based on the class results. Use your growth and color change results to conclude whether the unknown is a species of Staphylococcus, and whether it is potentially S. aureus. Unknown 1348 Date: h (+/-) Growt Pink/ Yellow Staphylococcus ? (Y/N) S. aureus ? (Y/N)
ASM 3. Based on your results, which organism(s) are likely to be a species of Staphylococcus? Explain your answer. 4. Imagine that you had growth on MSA plate, and the media changed from pink to yellow. What would be the clinical significance of this color change? 5. Mannitol salt agar contains 7.5% sodium chloride. What is the purpose of including such a high salt concentration in these plates? 6. For the MacConkey agar, fill in the chart below based on the class results. Use your growth and color change results to conclude whether the unknown is Gram negative, and whether it is potentially a coliform. Unknown (+/-) Growth white/pink Gram Negative? (Y/N) Coliform? (Y/N)
7. Based on your results, what do you know about each organism? 8. For the Eosin Methylene blue agar, fill in the chart below based on the class results. Use your growth and color change results to conclude whether the unknown is Gram negative, and whether it is potentially a coliform. Unknown (+/-) Growth white/dark/metallic green Coliform? Gram Negative? (Y/N) (Y/N)
9. Based on your observations which organism(s) is/are a coliform? Explain your answer. 10. Imagine you tested an environmental sample using MacConkey or EMB agar and you detected the presence of coliforms. What would be the public health/clinical significance of this result? 11. All 3 of the media we used are both selective and differential. Explain how these two terms differ.
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