- Hello Please Help Me With This Genetics Question 1 (159.09 KiB) Viewed 30 times
Hello! Please help me with this Genetics question???
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answerhappygod
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Hello! Please help me with this Genetics question???
Hello! Please help me with this Genetics question???
1. Suppose you have three Hfr strains of E. coli: A, B, and C. All are derived from a wild type prototrophic, str, F+ strain of E. coli. In separate experiments, you mate each strain to an F- recipient strain. Using an interrupted mating experiment, you determine the earliest time after mating that each of the markers can be detected in the F- recipient strain, as shown below. The data in the table are minutes. Maximal time of mating was 30 minutes. Gly + Lys + Nic + Phe + Tyr + Ura + HfrA x F- 6 0 9 2 3 HfrB x F- 13 2 0 9 10 HfrC x F- 15 2 12 19 18 Note: 0 means there was no transfer of that gene during the entire 30 min mating experiment. 5 12 16 a. Below is a circle that represents the bacterial genome. On this circle, draw the best map you can based on the available data. Mark the relative location of each of the 6 genes, the position and orientation of all 3 integrated Hfr plasmids, and the distance in minutes between each around the circle. Start with figuring out the order of the genes. Then figure out where the Hfr plasmids are situated between the genes. Then put them on the map and mark the distances between. See figure 16-4 in your textbook for an idea for how to mark the genes and Hfr orientations. The difference is that the book has separate maps for each Hfr strain, but here you will mark all Hfr positions and orientations on the same map.
1. Suppose you have three Hfr strains of E. coli: A, B, and C. All are derived from a wild type prototrophic, str, F+ strain of E. coli. In separate experiments, you mate each strain to an F- recipient strain. Using an interrupted mating experiment, you determine the earliest time after mating that each of the markers can be detected in the F- recipient strain, as shown below. The data in the table are minutes. Maximal time of mating was 30 minutes. Gly + Lys + Nic + Phe + Tyr + Ura + HfrA x F- 6 0 9 2 3 HfrB x F- 13 2 0 9 10 HfrC x F- 15 2 12 19 18 Note: 0 means there was no transfer of that gene during the entire 30 min mating experiment. 5 12 16 a. Below is a circle that represents the bacterial genome. On this circle, draw the best map you can based on the available data. Mark the relative location of each of the 6 genes, the position and orientation of all 3 integrated Hfr plasmids, and the distance in minutes between each around the circle. Start with figuring out the order of the genes. Then figure out where the Hfr plasmids are situated between the genes. Then put them on the map and mark the distances between. See figure 16-4 in your textbook for an idea for how to mark the genes and Hfr orientations. The difference is that the book has separate maps for each Hfr strain, but here you will mark all Hfr positions and orientations on the same map.