Questions 1. Why was it important to steam the slides in the Ziehl-Neelsen method) 2. Why is the acid-fast stain clinically important? 3. Which of the stained organisms was acid-fast? 89
4. The patient in the case file had to undergo a 4-month course of treatment. A typical course of treatment for a bacterial infection is 10 days. What does this suggest about mycobacterial diseases? Noncompliance is a major issue when treating TB because of the length of time of the treatment and the population typically exposed to the disease. What potential issues or problems does noncompliance pose in the area of public health? 5. The patient initially complained of pain in his hip and knee. A typical diagnosis for someone in this age range and with these symptoms would be osteoarthritis. Why did his physician assume a different cause? 6. You are viewing a clinical specimen that has been prepared using the acid-fast stain. All of the organisms are stained blue, and both cocci and bacilli are present. What can you conclude! Assuming the bacilli are mycobacterial cells, what procedural error occurred? 90
Interpretation and Interpretation and Questions 1. Why was it important to steam the slides in the Ziehl-Neelsen method) 2. Why is the acid
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