I’m planning to make an overexpression vector and I’m designing a primer for it. The problem is, I’m kinda confused with

[answerhappygod]

I’m planning to make an overexpression vector and I’m designinga primer for it. The problem is, I’m kinda confused with the primerdesign. The enzyme I’m going to use for the experiment is Xho andBamH1. The gene I’m going to overexpress is Srp1 from yeast. Cananybody confirm whether my primer sequence seems fine or not? Ineed to make a 29-mer primer.
<srp1 gene sequence>
ATGAGTCGCAGAAGCCTTCGTACCCTTTATGTGACAGGATTTCGAGATGGAATGCGTGCT
CGTGAGCTTGCTTATGAATTTGAGCCTTTTGGGCCTTTAATACGTTGCGACATCCCAATC
CCTAGAACTCGAACTTCAAGACCTTTTGCATTCGTTGAGTACGAAGATTCCCGTGATGCT
GAAGATGCGTATTATGAAGTGCACGGACGTCGTCTCGAAAGAGGTGGCGGTGTTCTTCGT
GTAGAATGGGCCAAGCAACCACCTCCGTCAGGCCCTGGCTCCAAACGTGGTGGAAGACGC
GAACGAGGAGGTCGTGTTCACGGAGATTCTGGACGGCTACGTTCAAGGTCACCTAGTCCG
CACGAAGCCCGATCTCGTTCCCCTTATAATGATGAACGATCTGATAGAAGATCAATGAGC
CCTCGTTATCGTTCGCGTAGTCGTAGTCCTGATGGACGTAGTCGGTCTCCCGATTATGAT
CGTCGTTCTCCTAAACGGAACCATCGTTCACCTTCACCAGTCTCTTTTGCACCTCAAAAG
AGCCCAGTAGAGAATGAAACTGAAACGAATGTTGATAATGGTGACACAAAAATCTCTGAG
TCTAACGAAAAATCTGGCACTGAAGTTGAACAACAATCTGCACCTAACTCTAATGGTAAC
GAGGAAGTTAATAATCTTGAACCAGTCGGTCAAAACGAAAGTAAGCAAGAGCCTCCTAAG
GAAGAGAACTCAAATGTCTCACAAGAGCAACCAGAACAAGCCCAACCCGAAGTGTCAGCT
GCTTCAGAGCAACCAGAATCCAACCCTA CTACTAC AGAGTCTCAATAA
Forward Primer : GCA CTCGAC ATGAGTCGCAGAAGC GGATCC
Reverse Primer : GCA CTCGAC TTATTGAGACTCTGA GGATCC