please explain and show work. design the following buffer - 450 mL of 175 mM alanine buffer at pH 3.00. You are to star

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please explain and show work.
design the following buffer - 450 mL of 175 mM alanine buffer at pH 3.00. You are to start with the fully protonated form of alanine. How many grams of NaOH will you need to add to create this buffer? MW of NaOH is 39.997 g/mol.
You are to design the following buffer - 450 mL of 175 mM alanine buffer at pH 3.00. You are to start with the fully protonated form of alanine. How many grams of NaOH will you need to add to create this buffer? MW of NaOH is 39.997 g/mol. Include your work in a separate document as instructed.
2.58 g NaOH
5.72 g NaOH
1.28 g NaOH
Alanine can't be used to make a buffer at this pH since it lacks a charged sidechain
0.576 g NaOH
the entire pepide were a single α-helix. With which two amino acids would the L closest to the N-terminus form hydrogen bonds to help create the α-helix?
Consider the peptide with the sequence SANTACLAUSISASTALKER. Assume this entire pepide were a single α-helix. With which two amino acids would the L closest to the N-terminus form hydrogen bonds to help create the α-helix?
Phineas and Ferb
I and T
T and U
N and I
S and R