Genetics Yeast Cloning And Sequencing Question 1 A Was I Successful At Isolating Plasmid Dna Please Explain B Was I 1 (44.52 KiB) Viewed 11 times
Genetics Yeast Cloning And Sequencing Question 1 A Was I Successful At Isolating Plasmid Dna Please Explain B Was I 2 (18.36 KiB) Viewed 11 times
Genetics Yeast Cloning And Sequencing Question 1 A Was I Successful At Isolating Plasmid Dna Please Explain B Was I 3 (32.6 KiB) Viewed 11 times
Genetics Yeast Cloning and Sequencing Question 1) a) Was I successful at isolating plasmid DNA? Please explain b) Was I successful at isolating recombinant plasmid? Please explain c) If I was successful, what are the approximate sizes of the yeast fragments cloned DIDELILI The first three lanes are loaded with the DNA Ladder, Undigested pUC19 and linear pUC19, respectively.* Lane 4 is "ECORI - Digested purified plasmid Colony 1" and Lane 5 is "Eco-R1 - digested purified plasmid from colony #2"
DNA Size Marker Undigested PUC 19 DeP/EcoRI/ pUC19 Group #1 samples Group #2 samples
1% TopVision" LE GQ Agarose #R0491) - - - - bp ng/0.5ug % 10000 30.0 8000 30.0 6000 70.0 14.0 5000 30.0 6.0 4000 30.0 6.0 6.0 6.0 3500 30.0 6.0 3000 70.0 14.0 2500 25.0 2000 25.0 1500 25.0 0.5µg/lane, 8cm length gel, 1X TAE, 7V/cm, 45min 5.0 5.0 5.0 1000 60.0 12.0 750 25.0 5.0 500 25.0 5.0 250 25.0 5.0
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