Aligning and calculating percent similarities for 10-base sequences is relatively easy, but as the number of bases being

[answerhappygod]

Aligning and calculating percent similarities for 10-basesequences is relatively easy, but as the number of bases beingcompared increases, it becomes much more difficult. The Basic LocalAlignment Search Tool (BLAST) is a powerful bioinformatics tooldesigned to compare much longer nucleotide or protein sequences ina matter of seconds.
BLAST is a publicly available program for searching knownnucleotide and amino acid sequences using several bioinformaticsdatabases. When you input a DNA sequence into BLAST, entire genomiclibraries are searched for identical or similar sequences. BLASTallows you to input either DNA or amino acid/protein sequences tosearch for other sequences that show similarities, or to comparesequence similarities between species. Thus, BLAST provides apowerful tool for linking gene or protein sequence information withfunction, and can be a useful tool for inferring the biologicalorigin of proteins.
Now you will learn how to use the BLAST tool in order to comparethe GAPDH nucleotide sequence from the leopard to the GAPDHnucleotide sequence from the lancelet. Follow the steps below:
1) Use a web browser to navigate to the BLAST homepage.
2) There are several options available for doing bioinformaticanalyses and sequence comparisons. Scroll down to the sectiontitled “Specialized searches” and click on the “Global Align”option.
3) Be sure that the "Nucleotide" tab is selected.
4) Select and copy the entire sequence below. It contains thecomplete nucleotide coding sequence for the GAPDH gene fromleopard. Be sure you select the entire sequence starting with ATGand ending with TAA.
ATGGTGAAGGTCGGAGTCAACGGATTTGGCCGTATTGGGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGGCAAAGTGGACATTGTTGCCATCAATGACCCCTTCATTGACCTCAACTACATGGTCTACATGTTCCAGTATGATTCCACCCACGGCAAATTCCACGGCACAGTCAAGGCTGAGAACGGGAAACTTGTCATCAATGGAAAGCCCATCACCATCTTCCAGGAGCGAGATCCCGCCAACATCAAATGGGGTGATGCTGGTGCTGAGTATGTTGTGGAGTCTACTGGGGTCTTCACCACCATGGAGAAGGCTGGGGCTCACTTGAAGGGTGGGGCCAAGAGGGTCATCATCTCTGCCCCTTCTGCTGATGCCCCCATGTTTGTGATGGGTGTGAACCACGAGAAGTATGACAACTCTCTCAAGATTGTCAGCAATGCCTCCTGCACCACCAACTGCCTGGCCCCTCTGGCCAAGGTCATCCATGACCACTTCGGCATCGTGGAGGGACTCATGACCACAGTCCATGCCATCACCGCCACCCAGAAGACCGTGGATGGCCCCTCTGGGAAGATGTGGCGTGATGGCCGAGGGGCTGCCCAGAACATCATCCCTGCTTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCTGAGCTGAATGGAAAGCTCACTGGCATGGCCTTCCGTGTCCCCACCCCCAATGTGTCCGTCGTGGATCTGACCTGCCGCCTGGAGAAAGCTGCCAAATACGATGACATCAAGAAGGTGGTGAAGCAGGCATCAGAAGGCACCCTCAAGGGCATCCTGGGCTACACTGAGGACCAGGTTGTCTCCTGCGACTTTAACAGTGACACCCACTCTTCCACCTTCGACGCTGGGGCTGGCATTGCGCTCAATGACCACTTTGTCAAGCTCATTTCCTGGTATGACAATGAATTTGGCTATAGCAACCGGGTGGTGGACCTTATGGTCCACATGGCCTCCAAGGAGTAA
5) Paste the leopard sequence into the “Enter Query Sequence”box in the BLAST window.
6) Repeat this by selecting and copying the complete nucleotidecoding sequence for the GAPDH gene from lancelet below. Be sure youselect the entire sequence starting with ATG and ending withTAG.
ATGCCTAAGGTCGGAATCAACGGGTTTGGTCGTATTGGCCGTCTGGTGCTGCGGTCTGCCGTTGAGAAGGGCCAGGAGGTGGTCGCCATTAACGACCCTTTCATCCAGCTGGACTACATGGTGTACATGTTCAAGTATGACTCCACCCATGGCAAGTTCAAGGGCGAGGTGTCTGCTGATGCTGACTCCTCTTCGCTGGTTGTCAACGGGAAGAAAATCAAGGTCTTCAATGAGATGAACCCAGCTAACATCAAATGGGCGGAAGCAGGTGCTGAGTACATCGTTGAGTCTACTGGTGTCTTTACCACCATTGCCAAGGCAAAGGCTCACCTTGATGCGGGAGCGAAGAAGGTCATCATCTCAGCTCCCTCTGCTGATGCGCCGATGTTCGTCATGGGTGTCAACCAGGAGAAGTACGAGAAGAGCATGGACATTGTCAGCAATGCTTCCTGCACCACCAACTGCTTGGCCCCACTGGCCAAGGTCATCAACGATGAATTTGGCATCGAGGAGGGTCTCATGACCACGGTACACGCCTACACCGCCACGCAGAAGACCGTGGACGGCCCGAGCGCAAAGGCCTGGCGTGACGGGCGCGGTGCCCACCAGAATATCATCCCAGCAAGCACTGGGGCAGCCAAGGCTGTGGGCAAGGTCATTCCTGAGCTGAACGGGAAGCTGACAGGCATGGCGTTCCGTGTGCCGGTCCCTGATGTGTCCGTGGTCGACCTGACCTGCCGACTGAAGAAAGGAGCTTCCTATGATGAGATCAAGGCTGTGGTGAAGAAGGCATCTGATGGCCCACTGAAGGGATATCTGGGATACACGGATGAGGATGTGGTGTCCTCAGACTTCATGGGCGACACCCACTCCAGTATCTTTGACGCCAAGGCGGGCATCGCACTGACCAACAACTTTGTCAAGCTTGTCTCTTGGTATGACAATGAATACGGCTACAGCTTCCGTGTTGTCGACCTCATCCAGTACATGGCCAGCAAGGAATAG
7) Paste the lancelet sequence into the “Enter Subject Sequence”box in the BLAST window.
8) Click on the “Align” button.
On the BLAST Results page, you will see the results of yourcomparison. Scroll down to the “Alignments” section to view thebase-by-base alignment of the two sequences. Here you will alsofind the “Identities” result. This value is the percent similaritycalculated as the number of matching bases at each position dividedby the total number of bases.
What is the percent similarity between the leopard GAPDHgene and the lancelet GAPDH gene? (Enter your answer as a wholenumber, without the percent symbol.)
%