- Given The Following Sequence What Would Be A Possible Grna On Either Strand For Cas9 Cas9 Recognizes Ngg Pam Seque 1 (20.35 KiB) Viewed 10 times
- Given The Following Sequence What Would Be A Possible Grna On Either Strand For Cas9 Cas9 Recognizes Ngg Pam Seque 2 (20.74 KiB) Viewed 10 times
- Given The Following Sequence What Would Be A Possible Grna On Either Strand For Cas9 Cas9 Recognizes Ngg Pam Seque 3 (21.39 KiB) Viewed 10 times
Given the following sequence, what would be a possible gRNA (on either strand) for Cas9 (Cas9 recognizes _GG' PAM sequences, where 'N' can be any nucleotide). 5'-TTGAAAGCTGTGTCTGTAAACTGATGGCT-3' 5'-GTCATCCTAGGCATATCGGG|AGG-3' TARGET 5'-AAGCTGTGTCTGTAAACTGA|TGG-3' TARGET | PAM TARGET 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3' IPAM TARGET IPAM 5'-AGTTCAGTCAAGTTTGCCTG| AGG-3¹ IPAM
Given the following sequence, what would be a possible gRNA (on either strand) for Cas9 (Cas9 recognizes _GG' PAM sequences, where 'N' can be any nucleotide). 5'-TACCTAGATGTTTTAACAGAAAAAGAAATAT-3¹ 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3' TARGET 5'-AGTTCAGTCAAGTTTGCCTG|AGG-3¹ TARGET IPAM TARGET Ⓒ5-CTTTTTCTGTTAAAACATCT | AGG-3' |PAM TARGET I PAM 5'-GTCATCCTAGGCATATCGGG|AGG-3¹ IPAM