- 2 Find The Paralogous Protein Of Baad0361 1 Explain And Define Your Answer With Reasons Explain The Steps Including 1 (76.85 KiB) Viewed 27 times
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2- Find the paralogous protein of BAAD0361.1. Explain and define your answer with reasons. Explain the steps (including which BLAST programme) you performed. 3- Identify the unknown protein by using below amino acid sequence. Give information about best match. How is the distance tree of first three hits? Have a prinstscreen of the distance tree. MNRRDFIKNTAIASAASVAGLSVPSSMLGAQEEDWKWDKAVCRFCGTGCGIMIARKDGKIVATKGDPAAP VNRGLNCIKGYFNAKIMYGEDRLVMPLLRMNEKGEFDKKGKFQOVSWQRAFDEMEKOFKKAYNELGVTGI GIFGSGQYTIQEGYAALKLAKAGFRTNNIDPNARHCMASAVVGFMQTFGVDEPSGCYDDIELTDTIITWG ANMAEMHPILWSRVSDRKLSNLDKVKVVNLSTFSNRTSNIADIENIFKPNTDLAIWNYIAREIVYNHPEA MDMKFIKDHCVFATGYADIGYGMRNNPNHPKFKESEKDTVEKENVITLDDEEATSLSYLGVKAGDKFEMK HQGVADKNWEISFDEFKKGLAPYTLEYTARVAKGDDNESLEDFKKKLQELANLYIEKNRKVVSFWTMGFN QHTRGSWVNEQAYMVHFLLGKOAKPGSGAFSLTGQPSACGTAREVGTFSHRLPADMVVANPKHREISEKI WKVPAKTINPKPGSPYLNIMRDLEDGKIKFAWVQVNNPWONTANANHWIAAAREMDNFIVVSDCYPGISA KVADLILPSAMIYEKWGAYGNAERRTQHWKQQULPVGAAMSDTWQILEFAKRFKLKEVWKEQKVDNKLTL PSVLEEAKAMGYSEDDTLFDVLFANKEAKSFNPNDAIAKGFDNTDVKGDERKIQGSDGKEFTGYGFFVQK YLWEEYRKFGLGHGHDLADFDTYHKVRGLRWPVVNGKETQWRFNTKFDYYAKKAAPNSDFAFYGDFNKML TNGDLIAPKDEKEHSIKNKAKIFFRPFMKAPERPSKEYPFWLATGRVLEHWHSGTMTMRVPELYRAVPEA LCYMSEKDGEKLGLNQGDLVWVESRRGKVKARVDMRGRNKPPVGLVYVPWFDENVYINKVTLDATCPLSK QTDFKKCAVKIYKA 4- By using the following cDNA sequence in Homo sapiens, search for the other DNA sequences to find one that encodes the same or similar protein. Which basic BLAST program you should use? What is the accession number of the possible match? Interpret the alignment result. ATGACTICTACCTGGTGATGCCCTTCAT GCAGACGGATCTGCAGAAGATCATGGGGATGGAGTTCAGTGAGGAGAAGATCCAGTACCTGGTGTATCAG ATGCTCAAAGGCCTTAAGTACATCCACTCTGCTGGGGTCGTGCACAGGGACCTGAAGCCAGGCAACCTGG CTGTGAATGAGGACTGTGAACTGAAGATT 5- Is there a 'creatine kinase B-type (Homo sapiens - NP_001814.2) ortholog represented in a genomic DNA database? Give details about your answer. Eg: Accession number, similarity percentage, expected value etc..