1. 2. What is the meaning of Vmax? What is the meaning of Km? Why are they important? A 0.5 M phosphate buffer at pH 6.5

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1 2 What Is The Meaning Of Vmax What Is The Meaning Of Km Why Are They Important A 0 5 M Phosphate Buffer At Ph 6 5 1 (120.57 KiB) Viewed 12 times

1. 2. What is the meaning of Vmax? What is the meaning of Km? Why are they important? A 0.5 M phosphate buffer at pH 6.5 and a 10 mM substrate stock solution are available for preparing enzyme assays. Each assay must have a total volume of 3 mL and a final phosphate concentration of 100 mM is desired. Using the substrate concentration of 3.33 mM and 100 µL of the enzyme solution, calculate the volume of buffer, volume of substrate stock solution, and volume of water that should be place in a cuvette for the assay. 3. For an enzyme kinetic experiment, it is recommended that the substrate concentrations extend from about 0.3 times Km, to at least 5 times Km. There should be at least 10 total data points, 5 of them having [S] above Km and 5 of them having [S] below Km. The data points should be spaced more closely at low [S], with at least one at high [S] approaching Vmax. If the Km of an enzyme is known to be about 1.5 mM, list 10 substrate concentrations that could be used for the assay series.