Part I. Select the one (1) best answer and record your response in the proper HU space on the answer sheet. If an "E" re

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Part I. Select the one (1) best answer and record your response in the proper HU space on the answer sheet. If an "E" response requires a response to be written, write the response in the space provided on the answer sheet. Failure to respond to a required written "E" response as identified will be considered to be incorrect. No partial credit will be given. 1. The biochemical characteristics used as an aid in identification of a bacterial organism B) whether the bacteria is motile are dependent upon A) the type of media used D) the staining C) the staining characteristics of the cell wall characteristics of cytoplasmic inclusions E) the presence of specific enzymes or non-motile 2. A) uses C) Fermentation differs from aerobic respiration in that only fermentation glycolysis as a pathway B) forms organic acids or alcohol end products releases energy from glucose D) uses ATP in the initial reaction E) none of the above = identify 3. In observation of a smear using the microscope, resolution is achieved through the use of A) the condenser B) oil immersion objective C) diaphragm D) either of the above E) none of the above identify 4. Before any microorganism can be identified, it must be A) isolated in pure culture B) tested with various chemical agents C) compared under the microscope to similar appearing organism D) all of the above E) none of the above = identify 5. Bacteria that forms resistant spores that enable the organism to survive adverse environmental conditions for extended periods of time belong to the following genera: A) Klebsiella and Treponema B) Mycobacteria and Actinomyces C) Bacillus and Clostridium D) Enterobacter and Pseudomonas E) all of the above 6. The primary reagent that is used to distinguish Staphylococcus from Streptococcus is A) oxidase B) Kovac's reagent C) citrated plasma D) hydrogen peroxide E) ferrous salts 7. The production of hydrogen sulfide from ferrous salts is most characteristic of A)) Enterobacter species - Escherichia coli B) Serratia - Providencia C) Staphylococcus - Neisseria D) Pseudomonas - Kelbsiella E) none of the above identify = 8. Bacteria movement is directly related to the presence of A) spores B) cilia C) flagella D) spokes E) neither of the above identify = 9. Production of the enzyme B-galactosidase and permease is most characteristically associated with the following: A) growth only along the line of inoculation on SIM media B) pink colonies on EMB C) hydrolysis of urear agar releasing ammonia D) production of indol upon the addition of Kovac's reagent E) none of the above = identify
B) failure to 10. A catalase positive gram positive cocci that produces a clot with citrated plasma is most characteristic of the following: A) growth on MacConkey's agar produce bubbles upon the addition of hydrogen peroxide C) contains the enzyme oxidase D) presence of enterotoxin in improperly stored or cooked food particularly meats or pastries E) none of the above identify 11. Incomplete lysis of sheep red blood cells with the production of methemoglobins A) a clear zone which is characteristic of products giving around bacteria colonies - Bacillus subtilis B) no zone around the bacteria colonies Escherichia coli C) a greenish zone around the bacteria colonies - Streptococcus mutans D) gamma hemolysis Staphylococcus epidermis E) none of the above = identify 12. A recombinant strain of Pseudomona syringae, developed using recombinant DNA technology, characteristically does not ferment sugars. Therefore this organisms appears as follows: A) red/pink or purplish colonies on EMB agar B) colorless colonies of MacConkey's agar C) tiny pin point colonies on blood agar D) growth only in the bottom of thioglycolate media E) none of the above identify 13. The mucoid characteristic of a bacteria colony is characteristically which usually produced by a bacteria such as strings when touched is best associated A) flagella - Proteus vulgaris B) cell wall - Corynebacterium species C) catalase production - Staphylococcus species D) capsule - Klebsiella species E) none of the above = identify 14. Protection of some bacteria against the humoral immune response and/or the inflammatory response in an infected host is achieved by the virulent (s) strains of Streptococcus pneumonae and by Klebsiella species by the A) slime layer B) cell wall C) pili D) capsule E) none of the above identify 15. Streptokinase, an enzyme that is used in cardiac therapy is produced by a microorganisms that is A) a gram positive bacilli B) a gram negative cocci C) a gram positive cocci D) either of the above E) none of the above = identify 16. On TSI agar, Shigella species produces a red slant and a yellow butt. Which of the following carbohydrates has been fermented? A) lactose B) sucrose C) lactose D) glucose E) all of the above 17. Rheumatic fever is a disease that results from the destruction of cardiac tissue by the immune system. This is a an example of A) reaction to antigen antibody tissue deposition by pyrogenic infections by Group A Streptococci B) natural immunity due to antibodies in nasal secretions C) autoimmune disease - IgG antibodies D) the clonal selection theory in action believed to be characteristic of b lymphocytes in antibody production E) none of the above =
18. Pseudomonas aeruginosa, a strict aerobic gram negative bacilli that is often cause of nosocomial infections in respiratory patients, is best characterized by the following: A) absence of growth on MacConkey's agar B) growth only at the top of the media interface in thioglycollate media C) retains the crystal violet iodine complex after decolorization D) all of the above E) none of the above = identify 19. A rapid procedure for determining the degree of sensitivity or resistance to a number of antibiotics on an agar plate is known as the A) Ames's test B) minimum inhibitory concentration (MIC) test C) the Kirby Bauer test C) broth dilution test E) none of the above identify 20. When animals and humans are subjected to drugs and antibiotics over long periods of time, the bacteria which may be associated with infections may acquire A) selective toxicity B) broad spectrum antibiotic susceptibility C) multiple resistant genes D) all of the above E) none of the above = identify 21. An exotoxin produced by a gram positive spore forming bacteria, which may be normally found in the soil, is best associated with the following A) flaccid paralysis B) rice water stools C) presence of IGA D) all of the above E) none of the above = identify 22. Rhizobium sp., a symbiont within the root nodules cells of legumes, is a gram negative aerobic rod that function in nitrogen fixation, is characterized by A) high teichoic acids presence in the cell wall B) formation of resist spores C) loses the crystal violet complex with decolorization D) stains with the counter stain potassium iodide reagent E) none of the above = identify. 23. Desulfovibnrio and Desulfuromonas are gram negative Bactria that are important because of their role in the anaerobic corrosion of iron in pipelines and heating systems. The following would be expected A) production of black precipitate with SIM media B) forms a complex with iodine and acetone alcohol C) stains with the primary stain safranin after decolorization D) all of the above E) none of the above = identify C) coco 24. One reason that immunity may not develop with many infections which may be caused by a variety of different microorganisms is due to failure of the microorganisms to stimulate A) a deficiency in the variable region of both light and heavy chains B) antigens C) memory B cells D) lysozyme E) all of the above
25. Eosin methylene blue agar (EMB) normally permits the growth of bacteria that contain A) high concentration of lipopolysaccharide and high murein concentration - Vibrio sp. B) high concentration on murein and low concentration of lipopolysaccharides-Micrococcus sp. C) low murein and low lipopolysaccharides Nesseria sp. D) low murein and high lipopolysaccharides - Salmonella sp. E) none of the above identify = 26. With urea agar, an increase in pH due to release of ammonia causes the indicator to turn pink to red is most characteristic of A) Escherichia coli B) Providencia sp. C) Salmonella sp. D) Proteus sp. E) none of the above = identify 27. Members of Yersinia, Kelbsiella, Shigella, Staphylococcus characteristically produce growth only along the line of inoculation in SIM or MIO agar. This suggests that these organism A) ferment lactose B) contain the enzymes to form hydrogen sulfide C) lack flagella D) contain 90% peptidoglycan E) none of the above = identify 28. Lancefields's group classification of a beta hemolytic Streptococcus sp. whose growth is inhibited in the presence of bacitracin is A) Streptococcus pneumonia B) Streptococcus viridans group C) Group B- Streptococcus agalactiae D) Group D- Streptococcus (Enterococcus fecalis) E) none of the above identify 29. In observation of a smear using the microscope, higher magnification requires while resolution is achieved through light through the use of the A) less adjustment of condenser-fine B) more - the use of the condenser-coarse adjustment C) more-diaphragm - fine adjustment D) less - diaphragm - condenser E) none of the above identify 30. Neisseria species and Pseudomonas species both oxidize certain aromatic amines such as tetra-methyl-p-phenylenediamine dihydrochloride, to a dark pink to purple end products. However the following is most characteristic of Nesseria A) a bacilli that retains the crystal violet complex after decolorization B) a cocci that stains with the counter stain safranin C) cocci that retains the crystal violet complex after decolorization D) bacilli that stains with safranin E) none of the above = identify 31. A family goes on a summer picnic. After playing ball for some time, they have lunch (ham salad, tomato salad, lemonade and apple pie) A few hours later they all become nauseated and begin to vomit. There is not fever. The next day they feel better. What type of food poisoning would you suspect? A) Salmonella B) Cholera C) Staphylococcal D) Botulism E) Parasitic
32. Kinases such as streptokinase and staphylokinases enable organisms to A) dissolve fibrin clots B) destroy blood cells C) destroy white blood cells D) dissolve tissue proteins such as collagen E) all of the above 33. What is the interpretation of a yellow butt and a yellow slant, with gas bubbles in a TSI slant? A) fermentation of sucrose and lactose with gas, sulfide negative B) fermentation of sucrose and/or lactose and dextrose with acid and gas, sulfide negative C) fermentation of sucrose, lactose and dextrose with sulfide formation D) non- fermentation of lactose, sucrose and glucose, sulfide positive E) none of the above = identify 34. The amino acid required in the medium used for the detection with p-dimethyl- aminobenzaldehyde reagent is A) oxidase B) lysine C) urea D) sodium citrate E) tryptophan 35. Streptococcus and Lactobacillus species, which are used to produce cheeses by starter cultures, are Gram stain B) gram A) positive cocci and gram positive bacilli C) gram variable cocci and bacilli identify negative cocci and negative bacilli fast positive E) none of the above D) acid 1 36. Acetobacter, a Gram is responsible for the commercial production of A) positive cocci - bread B) negative bacilli- amino acids C) negative cocci - fuels D) positive bacilli - yogurt E) negative bacilli-vinegar 37. Streptokinase, an enzyme that dissolves fibrin clots and is used in cardiac therapy, is produced by a microorganism that is A) a gram positive bacilli B) a gram negative cocci C) a gram positive cocci D) either of the above E) none of the above = identify 38. The utilization of oxygen as the terminal electron acceptor with the formation of water A) production of bubbles with hydrogen peroxide - characteristic of the following: C) Streptococcus sp. B) clumping of citrated rabbit plasma - Staphylococcus sp. cloudiness at the interface of the liquid and air layer in thioglycollate broth-Pseudomonas E) none of the sp. D) absence of growth on MacConkey's agar - Bacillus sp. above identify = 39. The genus of an oxidase positive gram negative cocci that is a normal inhabitant of the oral cavity is A) Bacteroides sp. B) Streptococci sp. C) Staphylococcus sp. D) Enterobacter sp E) Branhamella sp. 40. Lactobacilli, often used in the production of various cheeses and milk products, retains the crystal violet iodine complex after washing with acetone/alcohol. Therefore, this organism A) will produce colorless colonies of EMB B) will not grow on blood agar C) produces colorless colonies on EMB agar D) will not grow on MacConkey's agar E) none of the above = identify
Escherichia coli, often employed as an indicator of fecal contamination in environmental biology; and accounts for over 95% of urinary tract infections in females, utilizes carbohydrate fermentatively producing A) colorless colonies on blood agar B) lipopolysaccharide D) colorless colonies on EMB agar E) none of the above = identify C) contain greater than 90% absence of a periplasmic space in the cell wall 11. 42. Bacteroides fragilis, a gram negative anaerobic rod that is predominant in the GI tract A) produces endospores B) retains the primary C) will growth on blood and also inhabits the oral cavity stain after the acetone-alcohol rinse during Gram staining agar plates at 37C in the incubator D) grows in the bottom of thioglycolate broth E) none of the above = identify 43. Non-lactose fermenters on TSI agar correspond to the following: A) pink colonies on EMB agar C) green sheen on EMB agar C) colorless colonies on MacConkey's agar D) either of the above E) none of the above identify = same manner that the football or