Instructions Question: Would a kiwifruit and strawberry that are approximately the same size also have approximately the

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Instructions Question: Would a kiwifruit and strawberry that are approximately the same size also have approximately the same amount of DNA? Background: You have learned that DNA is carried on chromosomes. All mammals are diploid; however, not all plants are diploid. The common strawberry is octoploid (8n) and the cultivated kiwi is hexaploid (6n). Research the total number of chromosomes in the cells of each of these fruits and think about how this might correspond to the amount of DNA in these fruits' cell nuclei. Hypothesis: Hypothesize whether you would be able to detect a difference in DNA quantity from similarly sized strawberries and kiwis. Which fruit do you think would yield more DNA? Test your hypothesis. Perform the DNA extraction experiment. Record your observations: Because you are not quantitatively measuring DNA volume, you can record for each trial whether the two fruits produced the same or different amounts of DNA as observed by eye. If one or the other fruit produced noticeably more DNA, record this as well. Determine whether your observations are consistent with several pieces of each fruit. Analyze your data: Did you notice an obvious difference in the amount of DNA produced by each fruit? Were your results reproducible? Draw a conclusion: Given what you know about the number of chromosomes in each fruit, can you conclude that chromosome number necessarily correlates to DNA amount? Can you identify any drawbacks to this procedure? If you had access to a laboratory, how could you standardize your comparison and make it more quantitative? To complete this assessment, address all of the points above in a lab write up. Be sure to include images of your experiment in progress. See the examples contained here. Basic Requirements (the assignment will not be accepted or assessed unless the follow criteria have been met): . . . Assignment has been proofread and does not contain any major spelling or grammatical errors Assignment includes appropriate references. Assignment addresses the required points noted above (hypothesis, observations/data, analysis of data, conclusion). Need Help?
Which Has More DNA: A Kiwi or a Strawberry? Hands-on Laboratory: Competency 9 Performance Assessment Materials 900ml water 50ml liquid dish detergent 2 teaspoons table salt Kiwifruit Strawberries Either plastic bags or a mortar and pestle Cheesecloth and a funnel or tea baskets 2 thin-walled glass containers 2 large bowls or ice buckets, full of ice Ice-cold rubbing alcohol (put it in the freezer a few days in advance) Thin twine or string Procedure 1. Prepare a bottle of DNA extraction buffer from 900 mL water, 50 mL dish detergent, and two teaspoons of table salt. Mix by inversion (cap it and turn it upside down a few times). 2. Grind a strawberry and a kiwifruit by hand in a plastic bag, or using a mortar and pestle, or with a metal bowl and the end of a blunt instrument. Grind for at least two minutes per fruit. Add 10 mL of the DNA extraction buffer to each fruit, and mix well for at least one minute. 3. 4. Remove cellular debris by filtering each fruit mixture through cheesecloth or porous cloth and into a funnel placed in a clear glass container (a thin-walled juice glass works well). Place this glass in a bowl full of ice. 5. Pour ice-cold ethanol or isopropanol (rubbing alcohol) into the container. You should observe white, precipitated DNA. 6. Gather the DNA from each fruit by slowly swirling a piece of twine or string around in the liquid. You may also drop the twine in completely and then fish it out with a pair of tweezers.
0=X Figure 1: Mashed fruit and supplies ready In this image you can see I have mashed my fruit in Ziploc bags. I also already prepared my ice buckets and glass containers and have my other supplies waiting.
I Figure 2: Filtering kiwi Here I am filtering my kiwi. I chose to use tea baskets instead of cheesecloth and funnel. You can see the liquid collecting in the cold glass.
I Figure 3: DNA from strawberries This figure shows DNA precipitating from my strawberry extract. I poured in ice-cold rubbing alcohol and then dropped my string into the liquid. The froth forming is actually DNA.
Figure 4: DNA from strawberries C.C: D.O. Figure 5: DNA from kiw Figures 4 and 5 together show the different amounts of DNA I appear to have isolated from my strawberries and kiwifruit. I