You run a 2D gel and get the result shown. You notice pairs of spots that are on the same horizontal level (horizontal a

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You Run A 2d Gel And Get The Result Shown You Notice Pairs Of Spots That Are On The Same Horizontal Level Horizontal A 1 (562.58 KiB) Viewed 19 times

You run a 2D gel and get the result shown. You notice pairs of spots that are on the same horizontal level (horizontal arrows), but also two that are roughly the same size and near, but not exactly on, a vertical line with each other (angled arrows). Which technique was used to vertically separate the spots shown by the angled arrows, and what is their most likely relationship to each other? IEF, phosphorylated vs unphosphorylated IEF, RNA splicing variations of the same gene SDS, phosphorylated vs unphosphorylated SDS, unrelated proteins