Activity 3: Testing for the Presence of Protein in Solution Proteins Proteins are complex arrangements of amino acids. T

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Activity 3 Testing For The Presence Of Protein In Solution Proteins Proteins Are Complex Arrangements Of Amino Acids T 1 (49.61 KiB) Viewed 11 times

Activity 3: Testing for the Presence of Protein in Solution Proteins Proteins are complex arrangements of amino acids. The amino acids are linked to each other in long chains. In this exercise, a special compound, Biuret's reagent, will be used to detect proteins dissolved in solution. Materials 2 clean test tubes rack Dropper bottle of distilled (DI) water grease pencil Dropper bottle of 1% gelatin Biuret's reagent Procedure 1. Use the Sharpie or grease pencil to mark one test tube as "protein" and the other as "DI". 2. Add 10 drops of Biuret's reagent to each test tube. Record the color of the Biuret's reagent in the table below ("Reagent Color"). 3. Add 5 drops of 1% gelatin to the "protein" test tube. 4. Add 5 drops of distilled water to the "DI" test tube. 5. Gently shake each tube. 6. Wait a few minutes and Record the color of the sample in the table below ("Color after addition of sample"). Make note of any color changes that occurred. Color Reagent Color Sample after addition of samples (yes or no?) Test Sharpie marker or Dropper bottle of 7. In the "Conclusion" column, place a "+" if the tube is positive for protein or a "_" if the tube is negative for protein. 8. Use soap, water, and a test tube brush to clean the test tubes and place them in the rack to dry. Be certain they are not touching one another. Water 1% Gelatin (protein) tube What does the Biuret's reagent test for? What color indicates a positive reaction? Color Change Conclusion