stion 3 Read the case below and answer questions that follow: [11 marks] On the evening of June 19th, 2021 a husband and
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stion 3 Read the case below and answer questions that follow: [11 marks] On the evening of June 19th, 2021 a husband and
questions that follow: [11 marks] On the evening of June 19th, 2021 a husband and wife aged 55 and 45 years, respectively, bought tinned fish and consumed it for dinner The couple started to feel unwell and went to Charlotte Maxeke Hospital for consultation. Their condition soon worsened and they were admitted immediately. At first, the couple were vomiting, but soon symptoms of visual disturbances and weakness of the leg muscles later appeared. On the morning of June 21st, first the husband and then the wife died due to difficulty in breathing. a) In your opinion, what could be the cause of death for the couple? (1) b) What was the likely causal agent of their infection (Genus and spp)? Give reasons for your answer (2) c) After obtaining samples from faeces of the diseased, how would you enrich for the causal agent? (2) d) Following enrichment, you culture the bacteria in vitro and view the cells under the microscope ) what would be the shape of isolated bacterial cells?i) how would you determine the phylogenetic classification of these bactena and, i) what taxa and order would you expect them to belong to? (3) e) You want to determine the main virulence factor causing the boys symptoms using a systems biology approach. What i) 'omics' approach would you use? i) which technique and iii) what major virulence factor would be the most likely cause of the boys symptoms? (3) 2 out of 11 points
stion 3 Read the case below and answer