How many isotype controls you need to identify properly following population of cells – CD3 + CD8 + CD56 – CD16 – staine

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answerhappygod
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How many isotype controls you need to identify properly following population of cells – CD3 + CD8 + CD56 – CD16 – staine

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How many isotype controls you need to identify properlyfollowing population of cells – CD3 + CD8 + CD56 – CD16 – stainedwith 4 different fluorochromes?
a) 1
b) 2
c) 3
d) 4
Thanks. Please help with explain or reference.
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