- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 1 (29.9 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 2 (21.51 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 3 (21.51 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 4 (60.21 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 5 (60.21 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 6 (38.04 KiB) Viewed 9 times
- 1 04335337 Gi 73949907 Ref Xp 535320 2 Predicted Arginine Decarboxylase Isoformx1 Canis Lupus Familiaris 9 9517995 1 85 7 (50.7 KiB) Viewed 9 times
Step 1: Go to NGPhylogenyt to conduct a phylogenetic analysis. Choose A La Carte con Choose the following options Multiple Sequence Alignment Alignment Curation Tree Inference Tree Rendering Create Workflow Step 2: Upised the text file of your group to Input Data In MAFFT: Choose amino acids PhyML Nowick Do not change anything else GBlocks/BMGE: DetsType: Protein MAFFT Favor: One of the accuracy-oriented macacatalanm (see Note down your choice Choose BLOSUM45 BMGE or GBlocks PhyML (Maximum Ukelhood) Newick Display Figure 1 Model: Multiple Sequence Alignment Alignment Curation Tree Inference Tree Rendering Figure 2 Model: MAFFT GBlocks/BMGE PhyML Newick Do not change anything else Data Type: Amino acids Evolutionary Model: Choose one of these models: LG, WAG, JIT Tree Topology Search: Best of NN and SPR RESULT Note down the parameters you changed for each step in order here For each of the two evolutionary models, give the figures. Interpret your results: Interpret the tree, the relationship between OTUs and compare the models Statistical Test for Branch Support: Beetstrap: 500 times Branch Support depley branch support Branch Length: deplay branch length vacual cond Parameters
Step 1: Go to NGPhylogeny.fr to conduct a phylogenetic analysis. Choose A La Carte option. Choose the following options : Multiple Sequence Alignment Alignment Curation Tree Inference Likelihood) Tree Rendering Create Workflow. Step 2: Upload the text file of your group to Input Data. In MAFFT: Choose amino acids. Flavor : One of the accuracy-oriented (see ml) : MAFFT : BMGE or GBlocks https://mafft.cbrc.jp/alignment/softwar ... /manual.ht Note down your choice. Choose BLOSUM45. Do not change anything else. GBlocks/BMGE: PhyML : PhyML (Maximum : Newick Display Newick Data Type : Protein Do not change anything else. : Data Type: Amino acids Evolutionary Model : Choose one of these models: LG, WAG, JTT. Tree Topology Search: Best of NNI and SPR Statistical Test for Branch Support: Bootstrap : 500 times Branch Support: display branch support Branch Length: display branch length
RESULT Note down the parameters you changed for each step in order here. For each of the two evolutionary models, give the figures. Interpret your results: Interpret the tree, the relationship between OTUS and compare the models. Multiple Sequence Alignment Alignment Curation Tree Inference Tree Rendering MAFFT GBlocks/BMGE PhyML Newick Figure 1: Model : Parameters Figure 2: Model : : Interpretation Of Results
9.9517995 1.8546291 9.2928628 gi-62079257-ref-NP 001014283-1-Adc-gene-product-Rattus-norvegicus- gi-27370330-ref-NP 766463-1-antizyme-inhibitor-2-isoform-1-Mus-musculus- .62625864 1.1549199 gi-109001672-ref-XP 001106935-1-PREDICTED-arginine-decarboxylase-like-isoform-10-Macaca-mulatta- 1.4938668 >.0574163 gi-16506287-ref-NP 443724-1-antizyme-inhibitor-2-isoform-1-Homo-sapiens- 0.00917129 3.9447363 710032760-ref-XP 524555-4-PREDICTED-arginine-decarboxylase-Pan-troglodytes- 1.0536782 gi-84370045-ref-NP 001033599-1-ADC-gene-product-Bos-taurus- 491-513221567-ref-XP 004947906-1-antizyme-inhibitor-2-isoform-X1-Gallus-gallus- 1914511 038366282 3.2839962 0.1981879 gi-62858211-ref-NP 001015993-1-azin2-gene-product-Xenopus-Silurana-tropicalis-