"Silencing a silencer" (1.5 pts.) You are trying to determine whether a promoter, thought to have a role in tumor growth

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Silencing A Silencer 1 5 Pts You Are Trying To Determine Whether A Promoter Thought To Have A Role In Tumor Growth 1 (74.28 KiB) Viewed 94 times

"Silencing a silencer" (1.5 pts.) You are trying to determine whether a promoter, thought to have a role in tumor growth, is controlled epigenetically. You take DNA from normal cells and DNA from tumor cells and use a methylation-specific PCR approach. A methylation-specific PCR is below. If a gene has methylated cytosines or methylated cytosines, it can be determined by looking at the resulting bands. U = unmethylated detected, M = methylated detected a. 100% Unmethylated b. 100% Methylated M Based on the result from the experiment, state whether the promoter is being methylated to demethylated in the tumor cells. the promoter is methylated in the tumor cells The resulting methylation indicates that the gene under the control of this promoter is what type of cancer gene? the gene under control is a tumor suppressor gene You next decide to find out if there are other histone modification by running a ChIP assay. You isolate histones from WT cells and tumor cells that are in the DNA of the promoter you have been studying. You then separate the proteins and use antibodies detect whether they have methylation in histone protein H3 at lysine 4 or at lysine 9. No histone Histones from (control) WT cells MU 50% Methylated Promoter from Promoter from 50% Unmethylated normal cells tumor cells U M U M U M Histones from tumor cells Anti-H3K4me No histone Histones from (control) WT cells Histones from tumor cells c. Based on your results from (a) and (b), what can you say about the effects of methylating the lysines K4 and K9 on histone H3? Is it an activating expression change, a deactivating expression change or no net change in expression? Anti-H3K9me