The following bacterial species produce restriction enzymes with the indicated recognition sequences. Assume that each

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The following bacterial species produce restriction enzymes withthe indicated recognition sequences. Assume that each of thespecies has a modification enzyme that modifies the same sequenceas the recognition sequence for the endonuclease (as in the systemsyou learned about in class). Assume every possible site ismodified. Note that Y indicates a pyrimidine and R indicatesapurine. Bacillus stearothermophilus makesBstSNI with recognition sequenceTACGTA, Bacillus sphaericus makesBsuI with recognition sequence GTATCC,and Pseudomonas putida makesPpu21I with recognition sequence YACGTR.
a) You isolate genomic DNA from each species in a way thatmaintains all DNA methylation and then expose the isolated DNA toeach restriction enzyme under optimal invitro reaction conditions for that enzyme for long enoughto let the digest go to completion. For each reaction, indicatewhether all possible sites for the enzyme will be cut, some but notall possible sites for the enzyme will be cut, or none of thepossible sites for the enzyme will be cut.