In lipolysis, triglycerides are converted to glycerol and free fatty acids. During a lipolysis laboratory, we measured t

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In lipolysis, triglycerides are converted to glycerol and freefatty acids. During a lipolysis laboratory, we measured theconcentration of glycerol as a measure of lipolytic activity byreacting glycerol with reagents which resulted in the formation ofa red dye. The amount of red dye is in proportion to the amount ofglycerol. By measuring the amount of red dye in a sample, we wereable to calculate the amount of glycerol in the sample. How tomeasure the amount of red dye in a sample? In order to then be ableto calculate the glycerol concentration from the measurement of reddye in the sample, it is necessary to measure the amount of red dyealso in a so-called standard. Why is a standard needed here?