4 Stationary Phases In Lc Are Most Commonly Connected To Solid Support Particles Through A Covalent Linkage Here Are S 1 (78.94 KiB) Viewed 35 times
4. Stationary phases in LC are most commonly connected to solid support particles through a covalent linkage. Here are some common stationary phases: min min mhn Alkyl Phenyl Cyano a. Why do analytes interact with these stationary phases? List at least two specific IMFs.
b. The most common stationary phase is a C18 hydrocarbon chain covalently linked to a silica support particle, as seen below. CH3 Si O -Si — C18H37 Stationary phase (Silica particle) CH3 Mobile phase Si OH i. Would this be a normal phase or reverse phase? ii. What IMFs are available with this stationary phase? iii. Why would an analyte stop interacting with this stationary phase? iv. What could you do to get your analyte to stop interacting with this stationary phase?
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