Questions: 2a) Perform xtests on the five F2 datasets shown in your Excel file ('Question 2' tab). Your hypothesis in each case is that the resistance trait assorts independently from the marker trait. This time, you will need to organize and label the cells yourself. 2b) Which region of the chromosome is your resistance gene on? (e.g. between A & B,''between B & C', etc). Explain in 1-2 sentences how your results show this.
Controls Unknowns aja1 a1a2 aza2 Obs # 185 165 676, b1b2 Obs # 157 193 C1C1 C1 C2 C2C2 B Obs # 252 98 d2d2 didi didz Obs # 343 7 ene1 e1e2 eze2 Obs # 238 112
Table 3: Molecular marker crosses Parental cross: ?R? a bı ci di ei ?R? al bi ci di ei X ?r? az b2 C2_dz_ 2 ?r?az b2 C2 d2 ez F1 generation: ?R? ai bị C1 dị eh ?r?az b2 C2 d2 ez F1 test-cross: ?R? ai bi_ci_di_ei ?r?az b2 C2 d2 ez X ?r? az _b2_C2_d2 ez ?r?az b2 C2 d2 ez Segregating population F2 population: Resistant F2 plants are selected for before PCR analysis See your individual Excel file for the PCR data Table 3: Molecular marker crosses Parental cross: ?R? a bı ci di ei ?R? al bi ci di ei X ?r? az b2 C2_dz_ 2 ?r?az b2 C2
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