- Assay Of Phenindione In Phenindione Tablets By Uv Spectrophotometry According To Bp Content Of Phenindione C H O 92 5 1 (56.6 KiB) Viewed 98 times
Assay of phenindione in phenindione tablets by UV spectrophotometry according to BP Content of phenindione, C.H.O. 92.5-107.5% of the stated amount The stated amount of phenindione is 10 mg per tablet Assay Weigh and powder 20 tablets (total weight of 1.712 g), Shake a quantity of the powder equivalent to 50 mg of phenindione (active ingredient) with 150mL of 0.1M sodium hydroxide for one hour, add sufficient 0.1M sodium hydroxide to produce 250 mL, filter, and dilute 5mL of the filtrate to 250mL with 0.1M sodium hydroxide. Measure the absorbance of the resulting solution at the maximum at 278 nm. Calculate the content of C,H,O, taking 1310 as the value of A (1%, 1 cm) at the maximum at 278 nm. Abs 0.528