Dilution series Using a sterile pipette transfer 1 ml from the blender into 99 ml of sterile saline (1:100 dilution of t

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Dilution Series Using A Sterile Pipette Transfer 1 Ml From The Blender Into 99 Ml Of Sterile Saline 1 100 Dilution Of T 1 (48.93 KiB) Viewed 101 times

Dilution Series Using A Sterile Pipette Transfer 1 Ml From The Blender Into 99 Ml Of Sterile Saline 1 100 Dilution Of T 2 (54.15 KiB) Viewed 101 times

Dilution series Using a sterile pipette transfer 1 ml from the blender into 99 ml of sterile saline (1:100 dilution of the blender material) and shake to evenly disperse the organisms. (First dilution) Transfer 1 ml from the first dilution into 99 ml of sterile saline (1:100 dilution of the first dilution) and shake to evenly disperse the organisms. (Second dilution) 20 gm into l180 ml water 1. What is the total dilution in the first dilution (remember to take into account the dilution factor in the blender)? Write the dilution factor in scientific notation. What is the total dilution in the second dilution? 2. How many pipettes are needed to make this dilution series? The plating instructions are listed below. Draw the dilution and plating schemes for this experiment. Include the total dilutions represented at each plating. (You do not have to submit your drawing, but it may help you understand what you are doing). SPCA POUR PLATES Using a single sterile pipette transfer the following into individual Petri dishes Pipette 0.1 ml of the second dilution Pipette 1.0 ml of the second dilution Pipette 0.1 ml of the first dilution Pipette 1.0 ml of the first dilution Pour 20 ml of molten SPCA into each plate and suspend organisms by rotating the plates gently. VRBA POUR PLATES Using a single sterile pipette transfer the following into individual Petri dishes Pipette 1.0ml of the second dilution Pipette 0.1 ml of the first dilution Pipette 1.0 ml of the first dilution Pipette 0.1 ml of the blender material