Question 1 Extraction And Chromatography 1 1 The Following Data Apply To A Column For Liquid Chromatography Length Of 1 (46.63 KiB) Viewed 215 times
Question 1 Extraction And Chromatography 1 1 The Following Data Apply To A Column For Liquid Chromatography Length Of 2 (31.78 KiB) Viewed 215 times
Question 1 Extraction And Chromatography 1 1 The Following Data Apply To A Column For Liquid Chromatography Length Of 3 (31.78 KiB) Viewed 215 times
Question 1 [Extraction and Chromatography] 1.1 The following data apply to a column for liquid chromatography: Length of column=24.7 cm Flow rate = 0.313 mL/min VM = 1.37 mL Vs = 0.164 mL A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention Width of peak base (W) (min) time (min) Non-retained 3.1 A 5.4 0.41 B 13.3 1.07 с 14.1 1.16 D 21.6 1.72 Calculate: (a) The number of plates (N) from each peak [4] (b) The mean for N [2] (c) The plate height for the column [2] (d) The retention factor [2] (e) The partition coefficient (1) The resolution [2] 1.2 Briefly describe the variables that affect the column efficiency. [5] 1.3 A sample containing approximately 1 g of an organic substance A is dissolved in 100 ml. of water and transferred into a separating funnel. A 100 mL of ether was added and the content of the separating funnel was shaken well. Analysis of the ether layer shows that 0.7 g of A is extracted. (a) Calculate the value of KD=Ca/Caq for this system. [3] (b) How many additional extractions with 100 mL portions of ether would be required to extract 95% of the substance? [3] [Total marks: 25]
3. The following data were obtained on a 25.5 cm packed column. Flow rate = 0.325 ml/min, VM = 1.23 ml, Vs = 0.148 ml A chromatography of a mixture of species X and Y provided the following data: Retention time Width of peak (min) base (min) Nonretained 4.2 X 16.4 0.97 Y 18.2 1.06 Calculate: (a) The average number of theoretical plates in the column. [4] (b) The selectivity factor, a [3] (c) The resolution for X and Y. [4]
Register for solutions, replies, and use board search function. Answer Happy Forum is an archive of questions covering all technical subjects across the Internet.