ACTIVITY Disposal and Cleanup 1. Dismantle and clean the setup. Dispose of the contents in the sink with the water runni

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ACTIVITY Disposal and Cleanup 1. Dismantle and clean the setup. Dispose of the contents in the sink with the water running and rinse the tubes 2. Clean the workspace with soap and water. Data Tables Data Table 1: Gas Volume Observed at 15-Minute Intervals Initial 15 30 45 60 Total Glucose Sucrose Lactose Data Table 2: Moles CO./g x min) and Moles ATP/(g x min) for Each Sugar mL CO/Ig x min) Moles CO/(g x min) Moles ATP/(g x min) Glucose Sucrose Lactose
ACTIVITY ACTIVITY 1 A Fermentation of Monosaccharides and Disaccharides 1. With the grease pencil, label each of the three small bottles: one with Glucose, one with Sucrose, and one with Lactose. 2. Weigh 1 gram of sucrose in a weigh boat on the balance 3. Add the sugar to the appropriately labeled small bottle. Repeat weighing for each sugar. 4. Turn on a hot water tap and monitor the temperature with a thermometer until a 35 °C is achieved. Collect approximately 125 mL of 35 °C water in the beaker. 5. Weigh 2 grams of yeast, and add it to the 35°C water in the beaker Stir thoroughly with the spoon. 6. With a 10-ml graduated cylinder, add 6 mL yeast solution to each small bottle. 7. Layer approximately 1 cm of baby oil on top of the yeast solution in each bottle. Be careful not to mix the oil with the solution below. 8. Insert the bottle dropper tops on the bottles. 9. Turn on a hot water tap and monitor the temperature with a thermometer until a 35 °C is achieved. Fill three Styrofoam cups with 35 °C water. 10. Fill three 15-ml centrifuge tubes with 35 °C water. 11. Insert a piece of tubing into each of the centrifuge tubes, bend the tubing, and place a finger on top of the centrifuge tube. Quickly Invert the centrifuge tube and place it in the Styrofoam cup. Make sure as much water as possible stays in the centrifuge tube. The final result should resemble Figure 1. Make sure the end of the tubing is above the water level; if not, the water will empty out of the tube. Click here to see how to assemble the collection apparatus. http://players.brightcove. net/17907428001/HJ2y9UNi_default/index. html?videoldu4573398321001 12. Fill three more Styrofoam cups with 35 °C water. 13. Attach the tubes to the bottle dropper tops as shown in Figure 1. Insert the sugar and yeast-filled bottles into the Styrofoam cups with 35 °C water. 14. Record the initial gas volume in each centrifuge tube in Data Table 1. Figure 1. continued on next page 8 Carolina Distance Learning CAROLINA
ACTIVITY 2. A Fermentatic 1. With a grease Sucrose, one w Cellulose. 2. Add gram of s gram af starch of cellulose to th botties as desen 3. Attach the tubin described in Ac in Figure 1. Mai around the bort 15. Monitor the centrifuge tube gas volume for 60 minutes. Record the gas volume in Data Table 1 every 15 minutes. Make sure the water in the Styrofoam cups stays close to 35 "C. Add more hot water to the cups if necessary 16. After 60 minutes, record the total amount of gas produced for each bottle in Data Table 1. Should the tubes completely fill with gas before 60 minutes has elapsed, record the time it took for the tubes to fill and use this volume for the calculation 17. Calculate the volume of Co, gas produced for each gram of sugar as follows: mL Co,/gram (Start volume water tube-end volume water tube) Grams of sugar 18. Divide the number obtained from step 17 by 60 minutes or the time required to completely fill the tube. Record these values in Data Table 2. Note the differences between the different sugars used. 19. Using the data from step 18, calculate the amount of ATP produced. Divide the CO, gas volume calculated in step 18 by 24,000 mL/mole to get the moles of Co, produced. The moles of CO, produced are the same as the moles of ATP produced. Insert these numbers in Data Table 2 20. Dismantle and clean the setup. The bottle contents can be disposed of in the sink with the faucet running. Rinse the tubes, bottles, and tubing. 4. Note the gas va and record then Data Table 3. 5. Monitor the wate for 60 minutes. F minutes in Data in the Styrofoam more hot water 6. After 60 minuter of gas produced sugar as descrit 7. Use the formula calculate the minute for each Data Table 4. 8. Using the data amount of ATP Co, gas polumne 24,000 mL/mole produced. The the same as the Insert these nur CAROLINA www.car