You express CD14 as a chimeric construct fused to GFP in a macrophage cell line. CD14 is a GPI-anchored protein. Describ

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You Express Cd14 As A Chimeric Construct Fused To Gfp In A Macrophage Cell Line Cd14 Is A Gpi Anchored Protein Describ 1 (16.37 KiB) Viewed 211 times

You express CD14 as a chimeric construct fused to GFP in a macrophage cell line. CD14 is a GPI-anchored protein. Describe an experimental technique which would allow you to determine the rate of lateral diffusion of CD14 on the plasma membrane As part of your answer describe how you would be able to determine if CD14 was free to laterally diffuse or if its lateral movement within the membrane was restricted.