Question 3 4 points Save (1) You are provided with a 1.25 mM stock solution of p-nitrophenol (PNP) at pH 9.5. You also h

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Question 3 4 Points Save 1 You Are Provided With A 1 25 Mm Stock Solution Of P Nitrophenol Pnp At Ph 9 5 You Also H 1 (21.31 KiB) Viewed 143 times

Question 3 4 points Save (1) You are provided with a 1.25 mM stock solution of p-nitrophenol (PNP) at pH 9.5. You also have access to 0.1 M Borate buffer, at pH 9.5. Produce a table that shows the volume of stock and volume of buffer required to reach six concentrations of pNP between 0 μM and 1250 µM, usi a final volume of 2 mL. (2) The dilutions from part (1) are used to construct a standard curve. The absorbance coefficient that was measured from this standard curve for PNP is 18,000 M1 In a subsequent kinetics experiment you have measured Vo values of 1, 2, 2.5, 2.8, 3 and 3.1 min. Demonstrate how you can convert these valu from [min] to [M min-¹),