- Biochemistry Question 1 (258.29 KiB) Viewed 90 times
Biochemistry Question
-
answerhappygod
- Site Admin
- Posts: 899603
- Joined: Mon Aug 02, 2021 8:13 am
Biochemistry Question
Biochemistry Question
Q17-22 refer to the information below: ABCDE This is an image of a DNAse footprinting experiment testing how the small molecule IPTG effects the binding of a transcription factor to DNA. IPTG activates transcription at promoters containing the consensus sequence present in this DNA fragment. A: 76 basepair DNA fragment + DNAsel B: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor C: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor D: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor + IPTG E: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor + IPTG 17) Draw an additional lane next to lane A showing your predicted result if the scientist had NOT added DNAsel to lane A. (4 points)
Q17-22 refer to the information below: ABCDE This is an image of a DNAse footprinting experiment testing how the small molecule IPTG effects the binding of a transcription factor to DNA. IPTG activates transcription at promoters containing the consensus sequence present in this DNA fragment. A: 76 basepair DNA fragment + DNAsel B: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor C: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor D: 76 basepair DNA fragment + DNAsel +0.5 uM transcription factor + IPTG E: 76 basepair DNA fragment + DNAsel + 5 uM transcription factor + IPTG 17) Draw an additional lane next to lane A showing your predicted result if the scientist had NOT added DNAsel to lane A. (4 points)