Question 5 You are studying a protein that has 3 domains, but is only interested in domain 2. Describe an approach using

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Question 5 You are studying a protein that has 3 domains, but is only interested in domain 2. Describe an approach using

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Question 5
You are studying
a protein that has 3 domains, but is only interested in
domain 2. Describe an approach using PCR to clone domain 2
into the pcDNA3 vector, so that you
can express domain 2 as a recombinant protein.
Include a description of the PCR primer
design strategy in your answer. [10 marks]
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