Spectrophotometric Determination of Chlorophyll and Carotene Concentrations Calculations: 14.9259 Original mass of fresh

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Spectrophotometric Determination Of Chlorophyll And Carotene Concentrations Calculations 14 9259 Original Mass Of Fresh 1 (53.29 KiB) Viewed 34 times

Spectrophotometric Determination of Chlorophyll and Carotene Concentrations Calculations: 14.9259 Original mass of fresh spinach leaves Original volume of hexanes 51mL Volume of spinach extract added to column 5mL Concentration of Carotene in Spinach Size of volumetic flask used Absorbance at 450 nm Beer's Law A = £bc e is molar absorptivity (1.5 x 10' M'cm'¹) b is the path length (1.0 cm) c is the concentration (M) The total concentration of carotenes in the diluted sample (i.e. in the volumetric flask): C= A 0.297A/1.5x10 cm) (1.0cm)] = 1.98x10 m = eb Mass of carotenes in the fresh spinach leaves: Spinach 46279/ 900mL (462 7g) (50ml)( મbટ 1વ Mass % carotenes in fresh spinachº 50mL 0.297 A Experiment 7-18 mass carotenes x 100 mass spinach