During a test of kinetics of an enzyme-catalyzed reaction, the following data were recorded: E₂ T I S V (g/L) (°C) (mmol

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During A Test Of Kinetics Of An Enzyme Catalyzed Reaction The Following Data Were Recorded E T I S V G L C Mmol 1 (49.39 KiB) Viewed 35 times

During a test of kinetics of an enzyme-catalyzed reaction, the following data were recorded: E₂ T I S V (g/L) (°C) (mmol/mL) (mmol/mL) (mmol/mL-min) 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0.01 0.96 1.6 30 0.005 0.56 1.6 49.6 0.1 5.13 1.6 49.6 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0.0067 1.43 1.6 49.6 0.005 1.11 0.92 30 0.1 1.64 0.92 30 0 0.02 0.90 0.92 30 0 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.033 0.80 0.92 30 0.6 0.02 0.57 (a) Determine the Michaelis-Menten constant for the reaction with no inhibitor present at 30°C and at 49.6°C. (b) Determine the maximum velocity of the uninhibited reaction at 30°C and an enzyme concentration of 1.6 g/L. (c) Determine the K, for the inhibitor at 30°C and decide what type of inhibitor is being used.