Question 9 (6 marks) The cycle threshold (CT) value of quantitative real time polymerase chain reaction (qPCR) refers to
-
- Site Admin
- Posts: 899603
- Joined: Mon Aug 02, 2021 8:13 am
Question 9 (6 marks) The cycle threshold (CT) value of quantitative real time polymerase chain reaction (qPCR) refers to
Question 9 (6 marks) The cycle threshold (CT) value of quantitative real time polymerase chain reaction (qPCR) refers to the number of amplification cycles required for positive PCR results. It can be used as a proxy of the quantity of genetic materials in samples. Use your knowledge in qPCR, explain how the CT value could be used to reflect the initial amount of certain genetic markers (e.g., targeted genetic fragments of SARS-CoV-2 virus) in a sample. (6 marks)