b) Substances A and B have retention times of 16.50 and 18.20 minutes, respectively, on a 30 cm column. An unretained sp

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B Substances A And B Have Retention Times Of 16 50 And 18 20 Minutes Respectively On A 30 Cm Column An Unretained Sp 1 (32.8 KiB) Viewed 122 times

b) Substances A and B have retention times of 16.50 and 18.20 minutes, respectively, on a 30 cm column. An unretained species passes through the column in 1.30 minutes. The peak widths (at base) for A and B are 1.21 and 1.32 minutes, respectively. (6 marks) Calculate i) the time spent by A and B on the column ii) column resolution iii) average number of plates in the column iv) the plate height v) the length of the column required to achieve a resolution of 1.50 2[(tr):-(tx)] W,+W N = 16 46 ) (Rs),- _N (Rs), = VN, L H = N R W