I am working on a lab report and need some help understanding some details regarding LDH. What I think I understand: We

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answerhappygod
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I am working on a lab report and need some help understanding some details regarding LDH. What I think I understand: We

Post by answerhappygod »

I am working on a lab report and need some help understanding
some details regarding LDH.
What I think I understand:
We determine the rate of reaction of Lactate to Pyruvate, since
LDH binds to NADH, we can determine the rate of formation of NAD+
to NADH to calculate how effective the purification/recovery of the
enzyme was. The reduction of NAD+ creates a new maximum absorbance
peak at 340nm.
I would like more details on why we use 340 nm to
determine the activity of LDH?
any further details about this process is greatly appreciated,
thank you.
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