18) The following DNA sequence encodes part of the C-terminus of a prokaryotic protein. Use the codon table provided as

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18 The Following Dna Sequence Encodes Part Of The C Terminus Of A Prokaryotic Protein Use The Codon Table Provided As 1 (119.72 KiB) Viewed 15 times

18) The following DNA sequence encodes part of the C-terminus of a prokaryotic protein. Use the codon table provided as needed to answer the following questions (assume upper strand is sense): 5'-ATC-TAC-TGC-CAG-GTG-TAG-CTG-AGA-TGA-3' 3'-TAG-ATG-ACG-GTC-CTC-ATC-GAC-TCT-ACT-5' (A) Write the mRNA sequence that results from the transcription of this sequence. (1 pt) First letter of codon (5' end) Second letter of codon U с A G UUU Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys U UUA Leu UCA Ser UAA Stop UGA Stop UUG Leu UCG Ser UAG Stop UGG Trp CUU Leu CCU Pro CAU His CGU Arg CUC Leu CCC Pro CAC His CGC Arg с CUA Leu CCA Pro CAA Gin CGA Arg CUG Leu CCG Pro CAG Gin CGG Arg (B) Write the translated amino acid sequence that will appear in the C-terminus of the protein (1 pt) (C) How will the sequence change if a suppressor Tyr-tRNA with modified specificity that allows it to pair with G at its 5'-wobble position? (1 pt) AUU AUC lle ACU lle ACC Thr AAU Asn AGU Thr AAC Asn AGC Ser Ser A AUA lle ACA AUG Met ACG ThrAAA ThrAAG Lys AGA Arg Lys AGG Arg Val GCU Val GCC GUU GUC G GUA GUG Ala GAU AspGGU Gly Ala GAC Asp GGC Gly Ala GAA GluGGA Gly Ala GAG Glu GGG Gly D) If a mutation occurs that results in the deletion of G from the 3'-end of codon #5 how will this change the the C-terminal sequence of the protein? (1 pt) Val GCA Val GCG E) Explain how the degeneracy of the code limited the impact of deletion mutation introduced in question D? (1pt)